Abstract:

It’s an efficient way to get polyploidy by inducing the embryo sac chromosome doubling in Populus. In order to confirm the handling time of inducing the P. hopeiensis embryo sac chromosome doubling, development of embryo sac and zygote of P. hopeiensis were studied. The results showed that developmental process of embryo sac of P. hopeiensis was typical Polygonum type; the process not only was correlated with the development period, but also had a strong asynchronism. At the later period of embryo sac development and the process of zygote development, a large number of abnormalities were observed. Most mature and immature embryo sacs became vestigial, and developed into empty embryo sac or embryo sac with exceptional structure. Double fertilization and zygotic formation were infrequent. Taking into account the pollen of P. bolleana normal germination on stigma of P. hopeiensis, it suggested that embryo sac degradation might be the main reason for embryo abortion of P. hopeiensis.