Abstract:

In order to investigate the physiological effect the endophytic bacteria did on the plant, get the seeds without endophytic interference, in our research, we used the rape seeds as our material and we eliminated the natural endophytic bacteria of the rape seeds by dry heat treatment and moist heat treatment. Our results showed that, the treatment by dry beat sterilization at 50℃ for 3 days, or at 60℃ for 1-3 days could effectively eliminate the endophytic bacteria, the elimination rate could reach 99%, meanwhile, the seeds germination rate could reach 86%. But after moist heat treatment, the seeds germination rate was only 4%. We concluded that, dry hot treatment was the efficient method for eliminating the seed endophytic bacteria, and it would be the foundation for culturing the aseptic seedlings.