Abstract:

Rab2 is a member of small GTP-binding protein family controlling protein transport from endoplasmic reticulum to golgi body, some of them are found to be responsive to different environmental stresses. In order to obtain Rab2 in peanut, according to the jointed EST sequences, a pair of specific primer was designed, and Rab2 gene from peanut (AhRab2) was amplified successfully by RT-PCR. After T-A cloning, sequencing result indicated that the cloned cDNA contained a continuous complete open reading frame (ORF) of 636 bp encoding a polypeptide of 211 amino acids with a calculated molecular mass of 23.4 kDa. Sequence comparison of the AhRab2 revealed high level amino acid sequence identity with Rab2 from other species. Transcription of AhRab2 gene was differentially regulated by different environmental stimuli such as cold, dehydration, NaCl and the plant hormone ABA using real-time PCR. This gene can be used to regulate stresses resistance of peanut by genetic engineering.