Abstract:

To provide culture method for genetic transformation and tissue culture in vitro, efficient adventitious shoot induction and plant regeneration of embryonic leaflets among 18 peanut cultivars in 5 types were studied in this paper. The embryonic leaflets were cultured on MSB5 induction medium (Murashige and Skoog salts plus B5 vitamins) supplemented with 1 mg/L NAA and 6 mg/L BAP, and then transferred to the medium supplemented with 4 mg/L BAP. The result showed that the frequencies of bud point induction were different significantly in the cultivars tested that ranged 62.2%-95.5% as well as in 5 peanut types. The highest induction frequency was obtained in Arachis hypogaea var. hirsuta (87.2%), followed by Arachis hypogaea var. rulgaris (81.5%), the lowest was Arachis hypogaea var. fastigiata (63.1%). When the explants with bud points were transferred to the medium supplemented with 4 mg/L BAP, some of the bud points developed and then regenerated plants. The regeneration rate of plant was also different, the cultivars in Arachis hypogaea var. rulgaris produced higher frequencies (83.5%-97.1%), while that of Arachis hypogaea var. fastigiata produced lower frequencies (14.8%-22.0%).