Abstract:

In this study, it was initiated to establish an effective way to co-culture the bovine blastocysts and their uterine endometrium cells in vitro. Here, total 7 days in vitro fertilized (IVF) or 7 days parthenogenetic (PA) bovine blastocysts were put into the quadripuntal broad with p5 uterine endometrium cells. The results showed that, after being co-cultured with the uterine endometrium cells for 48 h, the hatching rate of IVF blastocysts was significantly higher than that of PA group (40±5)% vs (25±5)%, P＜0.05), but after being co-cultured for 72 h, there was no significant differences of hatching rate between them (70±8.66)% vs (65±5)%, P>0.05). In conclusion, these results suggested that such kind of in vitro co-culture system between those in vitro produced bovine blastocysts and the uterine endometrium cells, be a potential and effective way for further investigating the quality of bovine embryos.