Abstract:

To protect and utilize germplasm resources of wild and cultivated Dimocarpus longana Gour., from west Guangdong, RAPD method was occupied to study the genetic diversity of five varieties. 14 primers of good repetition and stability were screened from tested 100 RAPD primers. Among the 70 polymorphic bands obtained from 14 selective primers, 42 (60%) were polymorphic. The cluster analysis results showed that the genetic diversity was low level among the five cultivars, while the genetic distance between wild longan and other four cultivars was relatively far, 0.07375 on average. The wild longan formed a clade by itself, while the remaining four formed the second clade in the system tree, which indicated that the phylogenetic relationships between wild longan and any one cultivar were relatively far. The ‘Shuangmamu’ and ‘Dawuyuan’ were clustered together as the sister group with 0.01236 of the genetic distance, which suggested the both might have the same origin or be very close relationship cultivars.