Abstract:

Abstract：【OBJECTIVE】The surface disinfection is one of the critical steps during isolating the plant endophytes. The purpose of this article was to analyze and compare the affection of disinfection to the species and quantity of the endophytes isolated from walnut leaves. 【METHOD】The fungi were identified by the tissue isolation，the bacteria were identified by the dilution method.【RESULTS】The results indicated that the immersing 30S in 0.3% ClO2 was the best for isolating endophytic in walnut leaves, namely, five genera fungi and eleven species bacteria (including one actinobacteria) was isolated by ClO2, Three genera fungi and three species bacteria were isolated by NaClO, Three genera fungi and one specie bacteria were isolated by HgCl. 【CONCLUSION】This study also indicated that different disinfectants would isolate different endophytic groups. Anyone can not instead of others.