Abstract:

In order to determine the mixing concentration of auxin and cytokinin (NAA-BAP) which was conducive to the budding of Brassica campestris ssp. chinensis Makino var. parachinensis (Bailey) Tsen et Lee to help further agrobacterium-mediated transformation. This test uses MS medium added with mixing concentration of auxin-cytokinin has been used for cultureing cotyledon petioles of Brassica campestris ssp. chinensis Makino var. parachinensis (Bailey) Tsen et Lee, and also observed the budding. The results showed that mixing concentration of auxin-cytokinin have different effects on cotyledon petioles of Brassica campestris ssp. chinensis Makino var. parachinensis (Bailey) Tsen et Lee budding, when the mixing concentration of auxin-cytokinin for 0.5-1 mg/L, 0.25-2 mg/L were suitable for Brassica campestris ssp. chinensis Makino var. parachinensis (Bailey) Tsen et Lee budding. So cotyledon petioles of Brassica campestris ssp. chinensis Makino var. parachinensis (Bailey) Tsen et Lee budding affected by hormones also needs more extensive research.