Abstract: In order to research the Feasibility of Utilizing Corn Stalk, which was affluent but cheap, to process Conidial preparation of Trichoderma, fineness of corn stalk, moisture content, supplement of nutrient carbon sources and inorganic salts and stiring on the spore production were studied. The result showed that the fineness of corn stalk was 40-10 mesh, initial moisture was 60%-70%, and the spore production could reach 1.27×1010 spores per gram dry matter. Initial inoculum on the sporulation of Trichoderma had little impact. Add carbon sources and inorganic salts, the greatest spore production could reach 2.0×1010 spores per gram dry matter. But stiring was not conducive to sporulation. This showed that utilizing corn stalk to process conidial preparation of Trichoderma was feasible, efficiency, non-pollution, and it had important reference value to produce and promote Trichoderma agent.