Abstract:

In order to establish a rapid detection technique for mink Pseudomonas aeruginosa by Loop-mediated isothermal amplification. A set of specific primers were designed based on conserved region of PEA gene (Pseudomonas aeruginosa 240 bp), and amplified DNA for mink Pseudomonas aeruginosa by LAMP. The results were observed by turbidity, SYBR Green staining and agarose gel electrophoresis, meanwhile specificity and sensibility experiments were done too. The results showed that reaction was finished in 60 min, showed a high specificity and the detection limit was 1 ng/μL. The techniques were simple, rapid, high specificity and sensibility, so it could applied to base course and had comprehensive clinical prospect.