Abstract:

ADP ribosylation factor (Arf) GTPase-activating proteins (GAPs) promote the hydrolysis of GTP bound to Arfs to GDP, which plays a pivotal role in regulating Arfs by converting the active GTP-bound forms of these proteins into their inactive GDP-bound forms. The positive clone was screened from was screening and isolated from cDNA library of seedling leaves of pepper and was designated as CaAGD8. Sequence analysis showed that the 43.98 kD proteins encoded a polypeptide of 408 amino acid residues and contain an Arf GAP domain. The CaAGD8 with 73% and 71% similarity respectively to the AGD8-Like of grape and soybean and with 65% similarity to the AtAGD8. The real time PCR results showed that compared with control CaAGD8 relative expression was higher in stems, leaves, flowers and fruits of pepper.