Abstract:

The aim was to seek for a rapid DNA miniprep extraction method from wheat leaves. The wheat leaves were used as experimental materials, total DNA was extracted by using methods including modified CTAB, modified SDS and Boiling. Integrality and purity of nucleic acids were detected with agarose gel electrophoresis, ultraviolet absorption and PCR. DNA quality and purity extracted by modified CTAB method were high and had no degradation phenomenon. Two hundred DNA samples could be extracted each workday by per capita using this method. PCR detection of wheat transgenic plants showed that amplified bands of target gene were clear, without false-positive, and the test results were satisfactory. The DNA purity and concentration extracted by modified SDS method were not as good as modified CTAB method, but it also met the DNA requirements of major molecular research. The DNA quantity extracted by modified Boiling method was small and had a lot of impurities in it, PCR detection of this DNA showed no amplified band, the test results were unsatisfactory. Modified CTAB method provided a simple, rapid and miniprep method for extracting DNA from wheat, and was suitable for PCR amplification and other molecular biology research.