Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2013, Vol. 29 ›› Issue (1): 53-55.doi: 10.11924/j.issn.1000-6850.2012-2844

Special Issue: 园艺

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Study on the Growth Dynamics of Mulberry Hairy Roots

  

  • Received:2012-08-16 Revised:2012-09-24 Online:2013-01-05 Published:2013-01-05

Abstract: In order to establish the high efficient culture system of mulberry hairy root, the medium formula and culture conditions of mulberry hairy roots in submerged culture with shake flask were studied according to the index of the wet weight cultured 30 d. The content of total flavonoids in mulberry hairy roots was determined by spectrophotometry using rutin as standard substance. The results showed that the optimum medium was pH 6.0 MS culture medium, and the optimum culture condition was 26℃, 130 r/min, dark culture. The growth trends of mulberry hairy roots was“slow-fast-slow”, as“S”type curve, and the stable period was after cultured 21 days.