Abstract:

In order to establish in vitro culture and efficient regeneration of plantlet system of wine grape. The author used anther and stem tip of 5 wine grape varieties as explants and utilized the plant tissue culture method, the effect of 2 kinds of explants exogenous hormone, genotype on in vitro tissue culture and regeneration was studied. The results showed that: the effect of callus induction and differentiation of stem lip was better than anther. The stem tips cultured on B5 medium with 0.5 mg/L NAA and 1.0-2.0 mg/L 6-BA was advantageous to callus induction. The supplementation of 10 mg/L AgNO3 or 1000 mg/L PVP in above medium could better to stimulated callus formation of anther. The appropriate medium for differentiation of callus induced by the stem tip was B5 medium with 0.01 mg/L NAA, 0.5 mg/L 6-BA and 0.2 mg/L GA3. The survival rate reached to 100%. In this study, callus induction, shoots dedifferentiation with the stem tip of wine grape and efficient regeneration system of plantlet had been established, which could lay a good foundation for multi-propagation and gene transformation of wine grape.