Abstract: To establish a method which was use to detect the duck-derived components, one pair of primer was chosen to detect the duck in beef, fluorescent SYBR GreenⅠ Real-time PCR was developed for detection of the duck. The results showed that, duck gene DNA fragment could be detected and specially analyzed by melting curves, the detection limit was 1 pg DNA. Duck could be detected by the method from cooked duck and beef mixture. SYBR Green Ⅰ Real-time PCR was the effective methods for detecting duck.