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Chinese Agricultural Science Bulletin ›› 2013, Vol. 29 ›› Issue (11): 16-19.doi: 10.11924/j.issn.1000-6850.2012-2953

Special Issue: 畜牧兽医

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SYBR Green Ⅰ Real-time PCR for Detection of Duck-derived Components

  

  • Received:2012-08-29 Revised:2012-11-13 Online:2013-04-15 Published:2013-04-15

Abstract: To establish a method which was use to detect the duck-derived components, one pair of primer was chosen to detect the duck in beef, fluorescent SYBR GreenⅠ Real-time PCR was developed for detection of the duck. The results showed that, duck gene DNA fragment could be detected and specially analyzed by melting curves, the detection limit was 1 pg DNA. Duck could be detected by the method from cooked duck and beef mixture. SYBR Green Ⅰ Real-time PCR was the effective methods for detecting duck.

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