Abstract:

In order to explore the method for genetic quality control of closed colony, we studied the hereditary feature for KM and ICR mice in two departments of Liaoning Province by using microsatellites and viscera index detection. Detecting Index detection by conventional method, ten microsatellites loci on different chromosomes were chosen, and its polymorphism information content was rich, to analyze on genetic polymorphism of mouse by PCR amplification and PAGE electrophoresis. The results showed that, there was significant difference that body weight and part viscera index in two departments of Liaoning Province, and it was approximate about numbers of allele and genotype, H, PIC of ten microsatellites loci in two departments, genetic diversity of A departments KM was higher than B departments, genetic diversity of B departments ICR was higher than a departments, genetic distance was approximate between two departments of two strains. There was faint genetic differentiation in two departments of KM and ICR mice.