Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2005, Vol. 21 ›› Issue (6): 86-86.

Special Issue: 油料作物 园艺

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Efficient Regeneration from Peduncle Segments of Brassica napus L.

Han Dejun, Cao Li, Yuan Benwei, Chen Yaofeng, Li Zhenqi   

  • Online:2005-06-05 Published:2005-06-05

Abstract: Excised peduncle segments of three Brassica napus L. cultivars were used to optimize conditions for high-frequency shoot regeneration. The presence of 2,4-D(0.5~1.0 mg/L) in pre-cultured medium markedly enhanced the subsequent frequency of responding explants and the number of shoots/responding explant. The use of AgNO3 was a prerequisite for efficient shoot regeneration due to it strongly increased morphogenesis and decrease the frequency of explants death. BA (2.5mg/L) in combination with a low concentration of NAA(0.1mg/L) gave optimum shoot bud differentiation. The regenerated shoots could be rooted at a frequency of 95%.