Abstract: The aim of the present research was to determine early physiological and biochemical dynamic change in tomato seedlings inoculated arbuscular mycorrhizal (AM) fungi. Under greenhouse conditions total 3 treatments were designed: inoculation with Glomus mosseae(S), inoculation with Glomus etunicatum, Glomus mosseae and Glomus versiforme(M), and non-inoculation control(CK). The results showed that the colonization percentage, root length, the root of tomatoes fresh weight, leaf soluble protein content, POD and SOD activity in leaves in M were significantly higher than that of S, while the leaf chlorophyll content was lower in the former than in the latter. By single strain G. m invaded and by the group S, G. m+ G. e+ G. v composite strains infect M to evaluate the effect of infecting significant difference between the time period for CK group in eight days after infection, and a certain degree of sustainability. Predictably, AM fungi of tomato effect was infecting many aspects, single strain and compound strains infect effect had obvious difference. It is suggested that the physiological responds of tomato seedlings to early colonization by arbucular mycorrhizal fungi should be different from the responds in middle or later growth period. This needed to be further studied.