Abstract: The aim was to detect the expression of hepatocyte cytochr ome P450 2E1 in rat nonalcoholic steatosis model. Wistar rats were divided into normal group, hyperlipidemia model group, fermented Pu-erh tea group, Tieguanyin group, low, medium and high dose group of the black tea, which are 0.5 g/(kg?bw), 1.0 g/(kg?bw), 2.0 g/(kg?bw) respectively. All rats were sacrificed after 35 days’ feeding, serum levels of MDA, GSH-Px and SOD transaminase activity and pathological changes of the liver were observed. RT-PCR method was use to test P450 2E1 expression quantity in rat cells. Compared to the hyperlipidemia model group, the serum levels of MDA in low does group of the fermented Pu-erh tea were significantly decreased (P<0.05); Fermented Pu-erh Tea had a better effect on improving enzyme activity of GSH-Px and SOD than those of Tieguanyin and black tea; expression of hepatocyte cytochr ome P450 2E1 in high does group of fermented Pu-erh tea, black tea significantly decreased (P<0.05); through the specimens of rats and pathological detection. The high does group of fermented Pu-erh tea and Tieguanyin group could improve hepatic steatosis caused by high-fat diet significantly. Fermented pu-erh tea, Tieguanyin and black tea had different degrees of positive effect to the expression of hepatocyte cytochrome P450 2E1 in rat nonalcoholic steatosis model. And fermented Pu-erh tea had the most obvious effect.