Abstract: Using the stem of the Dendrobium officinale as explants, the effects of the MS medium with different concentrations of hormone on induction, proliferation, differentitation and taking root were studied. The result showed that MS+ 6-BA 0.5 mg/L+ NAA 1.5 mg/L was the suitable medium for the protocorm induction, the rate was 95%; MS+ 6-BA 1 mg/L+ NAA 1 mg/L was the ideal medium for the protocorm proliferation; MS+ 6-BA 5 mg/L+ NAA 1 mg/L was the best medium for the protocorm differentitation, the rate was 80%; MS+IBA 1.5 mg/L+ banana 100 g/L was the right medium for inducing the root growth, the rate was 100%.