Abstract: In order to explore the response characteristics of banana drought resistance to the suitable water, nitrogen, potassium interaction, the hydroponic experiment had been done. In this experiment, the leaf relative water content, root vitality, proline enzyme content, peroxidase activity changes of banana seedlings were studied and multiple comparisons analysis of the best drought effects combination of nitrogen and potassium concentration were done too, under different concentrations of nitrogen and potassium interaction by using PEG simulated drought stress. The results showed that: the impact of water stress on POD activity and Pro content of banana seedling leaves showed: PEG 30%>PEG 20%>PEG 10%>CK (0%). Analysis showed that: the interaction between the test factor: (1) water content of leaf size: PEG 5% & N 50 mg/L>PEG 15% & N 150 mg/L≈PEG 10% & N 100 mg/L; K 150 mg/L>K 200 mg/L>K 100 mg/L, and differences were significant between them. (2) Root activity of banana seedlings sequence: treatment 6 (PEG 10%, N 150 mg/L, K 100 mg/L)>treatment 5 (PEG 10%, N 100 mg/L, K 100 mg/L)>treatment 7 (PEG 15%, N 50 mg/L, K 150 mg/L). The root activities of the three treatments were significantly higher than the others, but there was no significant difference between them. (3) The proline content of treatment 9 (PEG 15%, N 150 mg/L, K 100 mg/L) was 2.16 times of treatment 1 (PEG 5%, N 50 mg/L, K 100 mg/L) of banana seedlings. Proline content of treatment 9 was significantly higher than those of other treatments. (4) The peroxidase activity performance of treatment 9 (PEG 15%, N 150 mg/L, K 100 mg/L) than treatment 1 (PEG 5%, N 50 mg/L, K 100 mg/L) was 1.46 times of treatment 1. Peroxidase activity of treatment 9 was significantly higher than that of other treatments. Appropriate concentrations of nitrogen and potassium could improve the leaf relative water content, root activity, proline content and peroxidase activity of banana seedling to reduce the adverse effect of water stress. In this experiment, treatment 6 (150 mg/L PEG 10%, nitrogen, potassium 150 mg/L) was the best appropriate treatment with water, nitrogen, potassium interaction on banana drought.