Abstract: The experiment was conducted to analyze the factors of influencing superovulation in Simmental Cattle, with the aim of increasing the availability of donor cows and accelerating industrialization of the embryo transplantation. The average number of recovered embryos and the average number of available embryos were taken as the main technical indicators, in agricultural areas of Hubei Province and pasturing areas of Inner Mongolia, the main factors of superovulation were compared, including primiparous and heifers, FSH of CAS(Chinese Academy of Sciences) and FSH of Canada, two different superovulation programs and different seasons. The results showed that: (1) The average number of recovered embryos and the average number of available embryos of heifers (9.8, 7.5) were higher than those of primiparous (7.5, 4.2) in agricultural areas (P<0.05), and those of heifers (10.3, 8.4) were higher than those of primiparous (8.0, 5.5) in pasturing areas (P<0.05). (2) There were no significant differences of the average number of recovered embryos and the average number of available embryos between the FSH of CAS (8.0, 4.8) and FSH of Canada (9.0, 5.9) in agricultural areas (P>0.05), and the similar result was acquired in pasturing areas, also no differences between the FSH of CAS (8.8, 5.5) and FSH of Canada (9.5, 6.5; P>0.05). (3) The Superovulation effects of program A (FSH+PG) and program B (CIDR+E2+P4+FSH+PG) were compared. The average number of recovered embryos and the average number of available embryos of program B (10.5, 8.0) were higher than that of program A (7.5, 4.8) in pasturing areas (P<0.05); and program B (9.6, 7.1) were higher than that of program A (7.0, 4.2) in agricultural areas (P<0.05). (4) The average number of recovered embryos and the average number of available embryos in summer (9.6, 7.5) were higher than that of in spring (6.0, 3.5) in pasturing areas (P<0.05). There were no significant differences of the average number of recovered embryos and the average number of available embryos between spring (8.2, 5.4) and autumn (9.0, 6.7) in agricultural areas (P>0.05). In conclusion: (1) Heifers could be the best choice as donors in agricultural and pasturing areas. (2) The FSH of CAS was better choice considering good superovulation result and low price；(3) The superovulation result in application of program B was better than that of program A. (4) For Pasturing areas, superovulation should be performed in summer, and for agricultural areas, superovulation could be performed both in spring and autumn.