Abstract: In order to study the multiple drug resistance of Salmonella and integron pop, this article from the Shandong Province farm for isolation of Salmonella species, according to the Kauffmann-White method for the determination of serum type, by microdilution method for the determination of the Salmonella 19 antimicrobial drugs against MIC, and using PCR technique for the determination of the class I integrons and carrying resistance gene cassette. The results showed, the isolated from 311 strains of Salmonella, including Indiana Salmonella (133 lines) and Salmonella Enteritidis (178 lines) in 2 serum type. Drug sensitivity test showed: isolated bacterial strains to sulfamethoxazole, sulfadiazine, nalidixic acid, ampicillin, tetracycline, doxycycline and trimethoprim resistance strains generally, multiple drug resistance rate was 91% (283/311 ), 99% of the isolates to Amikacin, polymyxin sensitive. PCR determination of integron results: class I integron detection rate was 65% (202/311), in which multiple phenotypic resistance strain type I integron positive rate was 92.6%, 202 isolates integrons positive strains in 6 strains carrying dfr17-aadA5 gene cassette. The results showed that, class 1 integron exists generally in Salmonella and related to multiple drug resistance.