Abstract: In order to assess corn gluten shelf life for a long time. The determination of corn gluten volatile matter to carry on the analysis by the electronic nose PEN3, using principal component analysis (PCA), linear discrimination analysis (LDA) detection estimate corn gluten shelf life for long time which divided into room temperature and refrigerated storage. The results showed that: room temperature samples sampling every day. occurring slightly metamorphic at the 4th day, occurring heavily metamorphic at the 7th day. Room temperature samples sampling every day, take sample 5 mL every week which stored refrigerated occurring slightly metamorphic at the 28th day, occurring heavily metamorphic at the 39th day.