Abstract: The evolutive status of morphological markers, cytological markers, biochemical markers and DNA molecular markers were summarized in the article. This paper mainly introduce DNA fingerprint techniques such as restriction fragment length polymorphism, random amplified polymophismic DNA, simple sequence repeats, amplified fragment length polymorphism, single nucleotide polymorphism and so on. This paper also studies the application of the classification and indentification of these techniques, the analysis and detection of the cultivars’ purity and the relationship among the cultivars on garden plants.