Abstract: Studies on the effect of plant hormones including 6-BA、NAA、and sucrose、CH on inducing Stevia Rebaudiana Bertoni’s Poly shoots using orthogonal experimental design. The results showed that the most important factor affecting on inducing caespitose shoots is sucrose, next is 6-BA、NAA, the lest one is CH. The best medium inducing poly shoots is MS+6-BA 1mg/L+ Agar6g/L+Sucrose30g/L. The developed medium is MS+6-BA 0.5mg/L+NAA0.05mg/L +Agar6g/L+Sucrose30g/L.The root shoots medium is 1/4MS+IBA 0.1mg/L+Agar6g/L +Sucrose 30g/L+active carbon1g/L, the rate of shooting root reach 100%. Now we have obtained many regeneration plantlets.