Abstract: The effect of follicular fluid (>10mm) on bovine oocyte maturation in vitro was evaluated by addition of different concentration (0, 10, 30 and 50%, respectively) of bFF to the maturation medium. A total of 1759 oocytes collected from abattoir ovaries were used. After 24h of maturation，the oocytes were activated by parthenogenesis(PG) or in vitro fertilization(IVF) and cultured ; Comparison of 10% bFF and 10% FBS with oocytes maturation; Efficiency of micro-drop culture was assessed. The results showed cleavage rates did not differ in parthenogenesis group (P>0.05), but embryo blastocysts rates was significantly greater than control (P<0.05), especially with 10% bFF; A significant decrease in cleavage rates (P<0.01) and blastocyst rates (P<0.05) was found with bFF concentration increase in IVF group. The rate of PG blastocyst and IVF blastocyst was the highest with the addition of 10% bFF (47.1% and 38.0%, respectively), more expanded blastocysts were gotten than others. In conclusion, addition of 10% bFF is benefit to the cytoplasmic maturation and can not hamper fertilization ability; bFF can be used in the IVM medium in place of FBS; Micro-drop culture do not increase the rate of the oocyte maturation.