Abstract: To reveal the pathway of MADS-Box transcription factor family regulating tomato fruit ripening, the research obtained tomato (Solanum lycopersicum) LeMADS- MC gene and LeMADS- antiMC gene by the polymerase chain reaction technique (PCR). The genes were connected to the plasmid pCAMBIA1300- 221 and pCXSN, and then transformed into Agrobacterium by Freeze-thaw method. Vectors were identified by PCR and enzyme digestion in the research and the sequence result was homology to NCBI database up to 100%. The results indicated that tomato expression vectors pCAMBIA1300- 221- MC and pCXSN- antiMC was constructed successfully. It would provide base for genetic research on fruit ripening and senescence through LeMADS-MC transgenic fruit.