Establishment of Leaf-explant Regeneration System in Vitro of Triploid Hybrids of White Poplar ‘Beilixiongzhu 1’and ‘Beilinxiongzhu 2’

doi:10.11924/j.issn.1000-6850.2013-2631

Abstract

Abstract: Abastract: To solve the difficulties in vegetative propagation of triploid hybrids of white poplar '‘Beilinxiongzhu 1’and ‘Beilinxiongzhu 2’, the effects on disinfection method and adventitious buds from different hormones combination were studied by taking semi-woody shoots came from triploid hybrids of white poplar ‘Beilinxiongzhu 1’and ‘Beilinxiongzhu 2’ as explants. The results indicated that: (1) the suitable method for disinfection of the two triploid hybrids was to treat for 20 minutes by 20% 84 disinfectant. (2)6-BA and TDZ had significant effects on regeneration rate and coefficient of the adventitious buds. However, NAA had insignificant effect on regeneration rate of the adventitious buds, but significant effect on coefficient of the adventitious buds. The optimum medium for the regeneration of adventitious bud from leaves in ‘Beilinxiongzhu 1’ was MS+ 0.2 mg/L 6-BA+ 0.1 mg/L NAA+ 0.005 mg/L TDZ. (3) Significant effects on regeneration rate and coefficient of the adventitious buds of 6-BA, NAA, and TDZ were observed in the triploid hybrid ‘Beilinxiongzhu 2’. The optimum medium for effective inducing adventitious buds in vitro leaves was MS+ 1.0 mg/L 6-BA+ 0.05 mg/L NAA+ 1.2 mg/L ZT. The rapid propagation systems of leaf explants in the two triploid hybrids were successfully established, which provided a platform for large-scale vegetative propagation and further genetic transformation of ‘Beilinxiongzhu 1’ and ‘Beilinxiongzhu 2’.

References

[1] 康向阳.关于杨树多倍体育种的几点认识[J].北京林业大学报, 2010,32(5):149-153.
[2] 康向阳,朱之悌,张志毅.银腺杨与毛新杨正反交三倍体选育[J].北京林业大学学报,2000,22(6):9-11.
[3] 朱之悌.毛白杨多圃配套系列育苗新技术研究[J].北京林业大学学报,2002,24(S1):4-44.
[4] 蔡国军,朱红斌,陈晓妮,等.三倍体毛白杨组培快繁和工厂化育苗技术研究[J].西北植物学报,2003,23(12):2188-2191.
[5] 郝贵霞,朱祯,朱之悌.毛白杨遗传转化系统优化的研究[J].植物学报,1999,41(9):936-940.
[6] Cai Xiao, Kang Xiang-Yang. In vitro tetraploid induction from leaf explants of Populus pseudosimonii Kitag[J].Plant Cell Rep 2011,30 (9):1771-1778.
[7] 刘乐承,白远国.芸薹属植物器官离体再生影响因素研究进展[J].长江大学学报:自科版,2006(2):175-178.
[8] Gary D Coleman, Stephen G Ernst. Shoot induction competence and callus determination in Populus deltoids[J]. Plant Science,1990, 71(1):83-92.
[9] 张存旭,张瑞娥,赵忠.不同无性系毛白杨离体植株再生能力比较研究[J].西北农林科技大学学报:自然科学版,2004,32(8):71-80.
[10] 李静,张景臣,郭伟,等.毛白杨叶片愈伤组织的诱导、芽的分化及不定根的再生[J].山东林业科技,2007,(01):52-53.
[11] 李慧,陈晓阳,李云,等.银白杨叶片不定芽再生影响因素的研究[J].北京林业大学学报,2004,26(3):46-50.
[12] 郑均宝,梁海永,孙克南,等.雄性毛白杨离体叶片再生及抗虫基因转化[J].河北林学院学报,1996,11(2):97-1011.
[13] Du Ningxia, Li Yun, Yu Haiwu. Establishment of high frequency regeneration system of Populus tomentosa[J]. Forestry Studies in China,2002,4(2):48-51.
[14] 李静,李志兰,梁海永,等.三倍体毛白杨叶片再生体系建立研究[J].河北农业大学学报,2006,29(4):48-52.
[15] Huang Dan qiong, Dai Wenhao. Direct regeneration from in vitro leaf and petiole tissues of Populu stremula‘Erecta’ [J]. Plant Cell Tissue Organ Culture,2011,107(1):169-174.
[16] Meiners J, Schwab M, Szankowski I. Efficient in vitro regeneration system for Vaccinium species[J]. Plant Cell Tissue Organ Culture, 2007,89(2-3):169-176.
[17] Koetle M J, Finnie J F, Staden J V. In vitro regeneration in Dierama erectum Hilliard[J]. Plant Cell Tissue Organ Culture,2010,103(1): 23-31.
[18] Confalonieri M, Balestrazzi A, Bisoffi S, et al. In vitro culture and genetic engineering of Populus spp: synergy for forest tree improvement[J]. Plant Cell Tissue Organ Culture,2003,72(2):109- 138.
[19] Magyar-Ta’ bori K, Dobranszki J, Teixeira da Silva J A, et al. The role of cytokinins in shoot organogenesis in apple[J]. Plant Cell Tissue Organ Culture,2010,101(3):251-267.
[20] 贾小明,樊军锋,王娟娟.河北杨和新疆杨离体叶片诱导不定芽研究[J].西北农林科技大学学报:自然科学版,2006,34(12):110-114, 120.