Abstract: In order to promote rational fertilization of walnut production in Shanxi ,Walnut trees were excavated, fractionated into various tree components at fruits harvesting stage to evaluate the character of dry matter composition and the properties of accumulation and distribution of N, P and K in different organs of Walnut (Juglans regia L.)trees which were 9 years old. The results showed that new organs accounted for 28.6% of the biomass of walnut tree，old organs for 61.5% and roots for 9.9%，respectively. The concentration of N, P and K of different organs has significant difference , and the nitrogen （N） content was the highest in the kernel, phosphorus（P） in the Kernel and potassium （K）in the shuck .Nitrogen was mainly distributed in the leaf， kernel， perennial branches and truck . Phosphorus was mainly distributed in the kernel，leaf，perennial branches and truck . Potassium was mainly distributed in the shuck and leaf. Amount of accumulation of N was highest , followed by K and P, and the ratio of N: P :K was 13.4:1:4.4, amount of accumulation of nutrients were higher in new organs than in old organs and roots.To obtain100 kg dry matter of fruits，Walnut trees must uptake N 10.3 kg， P 1.15 kg， K 8.76 kg.