Abstract: The TMV CP gene was synthesized with Polymerase Chain Reaction (PCR) y using the cDNA of its genomic RNA as a template. A synthetic ClaⅠsite was included in T5 primer while a BamHⅠsite in T3 primer, the PCR product was cloned into the ClaⅠ/BamHⅠ site of pBluescript KS+. Then bivalent plant expression vector, pKDTC (TMV-CP+CMV-CP), with double resistance to TMV and CMV, was constructed, in which each CP gene was controlled by a 35S promotor of CaMV. Transgenic tomato plants were regenerated via Agrobacterium transformation system, and analyzed by dot blot, PCR and Southern blot, these results showed that both genes were integrated into the transgenic plant lines L-2, L-5 of Lichun and JH-6 of Jinghan No 1. The transgenic plants grew normally and obviously resisted to the infection of corresponding viruses in greenhouse.