Abstract: This study was to focus on the sensibility of different rice species to acetochlor. Effects on rice sprout by acetochlor were studied by Petri dish method. The inhibitive effects on shoots, roots and fresh weights among different rice species were determined through the whole plant bioassay based on agar medium. The results showed that acetochlor had influence on the rice sprout. Acetochlor at low concentration could promoted the growth of rice shoots and roots, but it had strong inhibitory effect on them when acetochlor was applied at certain higher concentration and caused stronger inhibition to the roots than the shoots. Sensibility of different rice species to acetochlor was different. Among the sixteen rice species,‘Sidao’was the strongest resistant species, while‘Tianyou3301’the highest sensitive species. With application of 0.02 mg/L of acetochlor to rice seedlings, the inhibition ratio in shoots length, fresh shoots weight, roots length, fresh roots weight and roots number of‘Sidao’species were individually 1.46%, 6.42%, 51.06%, 60.87% and -6.45%, while those of‘Tianyou 3301’species 21.62%, 22.62%, 63.16%, 74.76% and3.89%. There was significant difference in the sensibility of rice among 16 different rice species to acetochlor and the correlation between the difference and species is irrelevant.