Abstract: This paper aimed to determine the contribution of leaves in different wheat varieties to grain, in order to provide theoretical basis for the stable high yield of wheat. Filed experiments were carried out on the Jianghuai region to study the effects of three treatments of removing leaf during anthesis on the chlorophyll content and grain yield in eight wheat varieties. The results showed that, the following: from the effects of removing leaf on the chlorophyll content, firstly, the effect of treatments of flag leaf on the chlorophyll content was in the following order: the treatment of removing other leaves except flag leaf > the treatment of removing 1/2 flag leaf > CK, but with the time passing away, the chlorophyll content of removing other leaves except flag leaf treatment was reduced, when reach the time of 20-25 days after anthesis the chlorophyll content was less than CK; The effect of treatments of backward second leaf on the chlorophyll content was in the following order: the treatment of removing flag leaf >the treatment of removing 1/2 flag leaf > CK, with the time passing away, the chlorophyll content of removing leaf treatments was higher than CK. From the effects of grain yield, the effect of grains per spike , the 1000-grain weight and grain weight per spike on the treatment of removing flag leaf was lower than CK, and was the lowest among all removing leaf treatments; The effect of the 1000-grain weight on the treatment of removing 1/2 flag leaf and the treatment of removing other leaves except flag leaf was lower than CK, but grains per spike were different among different wheat varieties, so grain weight per spike was also different. The effect of vary removing leaf during anthesis on ‘Liangmai 2’ and ‘Heng 7228’ is comparatively limited, so they are better for the stable yield of wheat.