Medical Value and Advances in Tissue Culture of Ginkgo biloba

Abstract

Abstract: In this paper, medical value in octolocular、leaf 、seed coat、kernel、timber of ginkgo and advances in tissue culture of are reviewed.The review of this paper is focused on tissue culture, such as stem culture, gametophyte culture, embryo culture, factors affecting the callus tissue formation and inhibition of browning during the culture. The basic culture medium that the ginkgo tissue culture is SH culture, main additional hormone of culture medium of induce callus are 2,4-D 2mg/L，6-BA1mg/L、NAA0.5mg/L and apple juice10%, under the condition that the strength of high voltage static electricity 1.0kV/cm and Cu2+ are to advantage the growth of callus. Improvement MS culture and paclobutrazol are contribitute to the sprout and differentiation of axillary bud . The MS cluture which is added BA2mg/L+NAA0.5mg/L、royal jelly and coconut milkcan induce ginkgo embryoid effectly。0.1% phytic acid、5.0mg/L ascorbic acid or 2000mg/L polyvinylpyrrolidone has the effect in inhibition of browning during the culture.

Fu Xiaoyi, Liu Guiru, Yang Xueju. Medical Value and Advances in Tissue Culture of Ginkgo biloba[J]. Chinese Agricultural Science Bulletin, 2005, 21(8): 314-314.

References

1 黄天芳,等.银杏资源开发现状[J].生物学杂志,1997,14(2):26~27
2 吴元立.银杏药用物质的开发利用及其发展设想[J].亚热带植物通讯,1998,27(2):52~55
3 梁立兴,等.我国银杏叶产品开发现状及前景[J].林业科技通讯,1999,(10):5~8
4 郑玉芝,等.银杏叶提取物及其在保健食品中的应用[J].食品工业科技,1996,(2):47~48
5 汪秋安.几类值得开发的天然黄酮类资源[J].湖南食品,1999,(2):18~19
6 曾华武,等.血小板激活因子和银杏内酯B对洗涤免血小板中cAMP含量的影响[J].中国药理学与毒理学杂志,1997,11(3):171~174
7 赵成林.银杏外种皮中酸性成分的提取与药用探讨[J].中草药,1997,28(4):250~251
8 许爱华,等.银杏外种皮多糖对不同状态小鼠血清SOD和MDA形成的影响[J].中国中药杂志,1998,23(12):746~747
9 梁立兴.我国银杏产业发展前景预测及战略调控[J].农业科技开发,1999,(3):6~7
10 曾新萍,黄志明.银杏综合开发利用.亚热带植物通讯,2000,29(3):58~63
11 郝岗平,等.银杏幼嫩茎段培养诱导愈伤组织及其细胞学研究.西北植物学报,2003,23(4):648~ 652
12 胡蕙露,等.银杏茎段组织培养正交试验[J].安徽农业大学学报,1997,24(2):129~133
13 王洪善,等.银杏愈伤组织培养形态建成的研究.[J].林业科技通讯,1997,9:27~28
14 徐利均,谢永红,甘霖,等.银杏组培繁殖及黄酮糖苷的产生[J].西南农业大学学报,2001,23(4):368~370
15 Carrier D J,Chauret N,Mancini M.Detection of gink gobiloba Ain Ginkgo Dilobacell cultures[J].Plant cell Reports, 1991,10(5):256~269
16 陈学森,邓秀新,章文才.培养基及培养条件对银杏愈伤组织黄酮产量的影响[J].园艺学报,1997,24(4):373~377
17 Fontanel A, Isabelle S, Vincent P. Regeneration in Ginkgo biloba LInduction of embryogenesis from mega gametophyte according to the developing stage[J]. Compets Rendus de Academic des Sciences Serie Ⅲ . Sciences de la Vie, 1994,317(2):149~155
18 Tulecjke WA.Thepollen of Gink gobiloba in vitro culture and tissue formation.[J].Amer.J.Bot,1957,44:602~608
19 Ball E. Growth of the embryo of Ginkgo biloba under experimental condition. I .Origin of the first root of the seedling in vitro[J]. Amer JBot, 1956,43:488~495
20 Ball E,lbid I .Effects of a longitudinal split in the tip of the hypocotyls [J]. Amer.J.Bot, 1956,43:802~810
21 Ball E. Growth of Ginkgo biloba under experimental condition. Growth rate of root and shoot upon media absorbed through the cotyledons[J]. Amer.J.Bot, 1959,46:130~139
22 王伏雄,陈祖铿,李宪章.银杏幼胚离体培养的研究I.蜂王浆对幼胚生长的影响[J].植物学报,1963,11(3):217~224
23 王伏雄,陈祖铿.银杏幼胚离体培养研究椰子乳汁对幼胚生长的影响[J].植物学报,1965,13(3):224~238
24 Li TT. The development of Ginkgo embryo in vitro.Contributions from the Botanical Laboratories of National Tsing Hun University, 1934.7
25 吴元立,严学成.银杏成熟胚培养的细胞组织学观察.林业科学,1998,34(4):8~13
26 罗言云,贾勇炯,曹有龙,等.银杏成熟胚的离体培养研究[J].四川大学学报,1999,36(3):573~577
27 Wetherell DF, Dougall DK.The Potassium requirment for growth and embryogenesis in a wild carrot suspension culture[J]. Physiol Plant, 1976,37:73~79
28 罗紫娟.银杏组织培养的初步研究[[J].云南植物研究,1984,6(l):93~97
29 Laurain D, Jean CC,Tremouillaux GJ. Direct embryogenesis from female haploid protoplasts of Ginkgo biloba L,a medicinal wood species .Plant cell reports,1993,12(11):656~660
30 David T W. Callus formation by Ginkgo biloba embryos on hormone free media controlled by closures and media components[J].Phylo-morphology, 1986,36(1):12~127
31 Carrier D J, Consentino G, N, Eufekd R. Archambault[J]. Plant Cell Reports, 1990,8:635~638
32 吴元立,严学成.银杏成熟胚乳培养的细胞组织学观察[J].果树科学,1998,15(4):327~331
33 吴元立,严学成.银杏成熟胚培养的细胞组织学观察[J].林业科学,1999,34(4):8~13
34 Laurain D, Chenieux J, Guiller T. Somatic embryo genesis from immature zygotic embryos of Ginkgo biloba[J]. Plant Cell Tissue and Organ Culture, 1996.44:19~24
35 徐刚标,何力,陈良昌.银杏愈伤组织诱导与继代培养的研究[J].中南林学院学报,1999,19(3):32~36
36 石贵玉,周巧劲,张振球.高压静电场对银杏愈伤组织生的影响[J].生物物理学报,1999,15(3):547~550
37 Santamour F S. Checklist of cultivated Ginkgo [j]. Arbori cuture, 1983,9(3):88~92
38 陈学森,张艳敏,董会.植酸在银杏组织培养中应用的研究[[J].天然产物研究与开发,1997,9(2):24~27
39 江静,尚富德,李锁平.银杏愈伤组织的诱导与激素优化组合的研究[T].河南大学学报,2000,30(3):51~54