Abstract: The acute toxicity properties of theaflavins and theaflavins tablet was carried out to evaluate by acute toxicity test method. The results showed that, the female mouse acute oral LD50 of theaflavins extracts was 2710.0mg/kg body weight, which was 136 times to 271 times of adultdaily dose (10mg/kg to 20mg/kg) . The male mice acute oral LD50 of theaflavins extracts was 3160.0mg/kg body weight, which was 158 times to 316 times of adult daily dose (10mg/kg to 20mg/kg). The female mice acute oral LD50 of theaflavins tablet was 20000.0mg/kg body weight, which was 400 times to 500 times of adult daily dose (40mg/kg to 50mg/kg) . The male mice acute oral LD50 of theaflavins tablet was 14700.0mg/kg body weight, which was 294 times to 368 times of adult daily dose (40mg/kg to 50mg/kg) . In a word, the male mice and female mouse acute oral LD50 of theaflavins extracts and theaflavins tablet were more than 100 times of adult dosage. It illustrated that the theaflavin extract and theaflavins tablet have less toxic, and was In accordance with the acute toxicity evaluation standards of health food.