Abstract: This research aimed at developing a plant regeneration system from base leaf, leaf and petiole explants of Anthurium andraeanum‘Dakota’and‘Alabama’, and to establish a foundation for mass production and transformation. Adopting the common tissue culture method, the regenerative ways were studied by analyzing the different differentiation ways through different explants on the medium of different hormone proportion. The results indicated that the callus induction rate of‘Dakota’from different explants showed as petiole＞leaf base＞leaf,‘Alabama’showed as leaf base＞petiole＞leaf. There were organ regeneration and somatic embryo regeneration way of‘Dakota’and‘Alabama’. On 6-BA 1.0 mg/L cultural condition, the rate of organ regeneration and somatic embryo regeneration of‘Dakota’were 91% and 54%, and that of‘Alabama’were 62% and 57%. The mixture regeneration way of‘Dakota’was 50%, and‘Alabama’was 57.1%. The germination percentages of embryonic calli on the 6-BA 0.5 mg/L and 1.0 mg/L medium of‘Dakota’were 55% and 46%,‘Alabama’were 71% and 66%. The research of‘Dakota’,‘Alabama’and three types of explants found regeneration modes had organ regeneration and somatic embryo regeneration. Organ regeneration was more important in the early stage, and somatic embryo regeneration was more important in the late stage.