Establishment of Leaf Regeneration System in Ginseng Fruit‘changli’

doi:10.11924/j.issn.1000-6850.2014-0191

Abstract

Abstract: In order to establish the genetic transformation regeneration system of ginseng fruit, taking the seedlings leaves of‘Chang Li’as test material, the effects of types of culture medium, pH and hormones on the adventitious buds subculture proliferation of ginseng fruit were studied. The results indicated that MS medium was favorable to the tube seedling of ginseng fruit and the average plant height was 8.5 cm cultivated for 24 d. GS medium was the optimal condition for the root generation of the tube seedlings, and the number of root generation could reach 13.8 per shoot. 6- BA blended with TDZ could improve the generation rate of adventitious buds. The regeneration ratio of leaves was 83.3% on the MS+6-BA 2.0 mg/L+TDZ 0.5 mg/L medium, the regeneration coefficient reached 8.5. Therefore, MS + 6- BA 2.0 mg/L + TDZ 0.5 mg/L was the optimal medium and in favor of the adventitious shoot regeneration of leaves.

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