Abstract: The aim of the study was to provide a theoretical basis for the configuration of ratio of row spacing to intrarow spacing (RS/IS). With early rice‘Zhongjiazao 17’and‘Ganxin 203’and late rice‘Ganxin 688’and‘WufengyouT025’as materials, the effects of RS/IS (Ratio of row spacing to intrarow row spacing) on yield, nitrogen accumulation and utilization were studied in the same planting density of 31.20×104 hills/hm2. The results showed that yield of early rice were higher in RS/_2.8 (30.0 cm×10.7 cm), RS/_2.0 (25.0 cm×12.8 cm) treatment and lower in RS/_5.0 (40.0 cm×8.0 cm), RS/_1.3 (20.0 cm×16.0 cm) treatment, while for late rice was higher in RS/_5.0, RS/_2.0 treatment and lower in RS/_2.8, RS/_1.3 treatment. For early rice, the total nitrogen accumulation and apparent utilization ratio of nitrogen were higher in RS/_2.0 treatment and lower in RS/_5.0 treatment, while for late rice, that were higher in RS/_2.8 treatment and lower in RS/_5.0 treatment. For early rice, nitrogen requirement for 100 kg grain production was higher in RS/_1.3 treatment and lower in RS/_2.0 treatment, while for late rice that was higher in RS/_2.8 treatment, lower in RS/_5.0 treatment. It was obvious that 4 varieties of early and late rice obtained higher yield in 25.0 cm×12.8 cm and lower yield in 20.0 cm×16.0 cm, and treatment 40.0 cm×8.0 cm had lower total nitrogen accumulation, nitrogen requirement for 100 kg grain production and apparent utilization ratio of nitrogen.