ISSR Analysis of Genetic Diversity of Eggplant (Solanum melongena L.) in Southeast Asia

doi:10.11924/j.issn.1000-6850.2014-0488

Abstract

Abstract: To clarify genetic diversity and relationship among eggplant germplasm, 18 accessions of eggplant including 10 from Southeast Asia, 3 from South America and 5 from China were assessed with ISSR markers. The results showed that 433 loci were amplified by 17 ISSR primers, and 390 loci were polymorphic bands, the percentage of polymorphic loci was 92%. The average observed number of alleles, average effective number of alleles, average Nei’s gene diversity index, and average Shannon’s information index were 1.8971, 1.4778, 0.2850, and 0.4336, respectively. The genetic similarity among all accessions ranged from 0.55 to 0.81. The 18 accessions of eggplant were clustered into two groups by UPGMA when GSC was 0.67, 10 accessions from Southeast Asia were clustered in group I, 3 accessions from South America in group II. The result showed that there was rich genetic diversity in eggplant cultivars between those from Southeast Asia and those from China and South America, provided a theoretical basis for further research and breeding of eggplant.

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