Effect of Fertilizer on Tea Plant Rhizosphere Bacteria Diversity

doi:10.11924/j.issn.1000-6850.2014-0605

Abstract

Abstract: Denaturing gradient gel- electrophoresis (DGGE) method was employed to determine tea plant rhizosphere and body soil bacteria community diversities with soil pH and C/N, to investigate the effect of different fertilization treatments. More detailed analysis of 16S rRNA gene sequences to determine the phylogenetic position of specific strains was conducted. In addition, root auxin (IAA) quantity was detected by colorimetry. Results showed that organic fertilizer could significantly increase soil C/N, which was positively correlated to bacteria community, and the correlation coefficient (r) was 0.93 (P＜0.05). Among different treatments, bacteria diversity was the highest in the organic and chemical fertilizer (7:3) treatment (rhizosphere) and (95:5) treatment (soil) respectively. Bacteria 16S rRNA band gene sequences showed both chemical fertilizer and organic fertilizer would affect the efficiency of specific bacterial colonization. The results also showed the highest bacteria diversity treatment also contained the highest content of IAA in tea root. In conclusion, organic fertilizer can elevate soil C/N and microbial community diversity and bacterial diversity has positive relationship to soil C/N.

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