Abstract: In order to study the suitable nitrogen application and the influence of dry matter accumulation and N absorption under different N application levels on spring maize, field experiment was conducted under different N application rates (N 0 kg/hm², 120 kg/hm², 240 kg/hm² and 360 kg/hm²). The results showed that both the dynamic dry matter accumulation and N absorption of spring maize could be described by Logistic equation. N application rates could significantly influence both the maximum rates of dry matter accumulation and N absorption, and the order of different N application rates was N₃₆₀＞N₂₄₀＞N₁₂₀＞N₀. The results also showed that maize yield increased with the increment of nitrogen application rate. When the nitrogen application rate was 360 kg/hm², the maize yield reached the highest 10081.5 kg/hm², but it had no significant difference with N₂₄₀ and the nitrogen utilization efficiency was 15.5% lower than that of N₂₄₀. Considering the maize yield and nitrogen utilization efficiency under the experimental conditions, N application rate of 240 kg/hm² was identified to be the optimum amount.