Advances in the Research of N-glycosylation of Recombination Pharmaceutical Glycoprotein Expressed in Transgenic Plants

Abstract

Abstract: The research for plants has been a hot spot for more than ten years long as the bioreactor to produce recombinant pharmaceutical proteins. The number of therapeutic proteins successfully produced in plants is steadily increasing and is expected to grow even more rapidly in the future. More than 100 recombinant proteins were expressed in transgenic plants. Some of them have been produced for the commercial purposes. It has been widely testified that recombination therapeutic proteins expressed in transgenic plants can be produced successfully and retain their biological activities largely. The protein synthesis and folding mechanism in plant and animal cells are conserved very much, however, differences observed in post-translational modification between the plants and mammals have been widely concerned. Because most pharmaceutical proteins expressed in transgenic plants are secretory proteins and many secretory proteins are glycoproteins. so N-glycosylation of recombination proteins in transgenic plants have been being closely concerned. As a result, to reduce the immunogenic possibility of recombination proteins many strategies have been tried to humanize the recombination proteins expressed in transgenic plants systems. Advances in these Researches are essential to explore the potential of plants and expand their utilization scales as the bioreactor. Advances on both the N-glycosylation of therapeutic glycoproteins produced in transgenic plants and current strategies to humanize their N-glycosylation are discussed in this review.

Wang Yang, Cui Jizhe, Zhou Jing, Cui Caixia, Sun Yugang. Advances in the Research of N-glycosylation of Recombination Pharmaceutical Glycoprotein Expressed in Transgenic Plants[J]. Chinese Agricultural Science Bulletin, 2005, 21(10): 174-174.

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