Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2005, Vol. 21 ›› Issue (10): 19-19.

Special Issue: 生物技术 畜牧兽医

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Construction of Goat Genomic Library

Tang Shuang, An Zhixing, Zhang Mingfeng, Song Hongwei, Zhang Yong   

  • Online:2005-10-05 Published:2005-10-05

Abstract: Goat genomic DNA library was constructed for screening milk-protein-encoding gene of goat and construction of knock-in vector. Genomic DNA was prepared from blood sample, partially digested with Sau3AΙ.15!23kb fragment was fractionated and ligated to Lambda BlueSTAR BamHΙ Arms. Titer of the library is 3.4×106; 1.7×106 clones were obtained with inserts size ranging from 15~23kb.