Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (1): 43-46.doi: 10.11924/j.issn.1000-6850.2014-1668

Special Issue: 生物技术 园艺

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Establishment and Optimization of Multi-gene Transformation system of Populus

  

  • Received:2014-06-12 Revised:2014-11-04 Accepted:2014-11-24 Online:2015-03-18 Published:2015-03-18

Abstract: In order to establish and optimize the multi-gene transformation system of Populus. ‘Ningyang No.1’ was used as acceptor material, and multi-gene transformation system was established. SOS1, SOS2 and SOS3 genes related to salt resistance were co-transformed. The results showed that the most suitable medium of adventitious bud was MS 6-BA 0.5 mg/L NAA 0.2 mg/L, the most suitable screening concentration of herbicide for the leaf piece was 0.8 mg/L, and the most suitable Agrobacterium suspension concentration was OD600=0.4. The 14 lines of PCR positive were obtained using this system.