Abstract: To develop the effective preparation methods of pollen suspension for kiwi spray pollination, the mixed pollen and 'Yumihoutao 2' as materials, was carried out by using crude pollen preparation pollen suspension test and liquid pollination test. The test results showed that the pollen concentration of the suspension increased along with the increased of amount of crude pollen added. Crude pollen adding amount of 12%-16% (w/v), the pollen concentrations of the suspension of up to 1.25-1.75 million/mL, and the pollen germination rate were more than 85%, and there was no significant difference with controlled trials (add 5% of refined pollen); the fruit setting rate be almost 100% each treatment. More than 12% of the test treatment of fruit weight was about 100 g, the seed number was 1100-1220, and controlled trials there was no significant difference between the above treatments. Fruit weight, seed treated by 4% test were significantly lower than the above treatments. Soluble solids content and acid content there was no significant difference between different test treatment. Light absorption index of suspension has a close correlation relationship between pollen concentration (r=0.98), absorption index of 2.5 can be used as judgment index of pollen concentration be appropriate or not. This method omitted the pollen refined process, was a kind of kiwi artificial pollination technique efficient labor-saving.