Detection of Cucumber Mosaic Virus Infecting Vegetable Crops Grown in Beijing Suburban Greenhouses

doi:10.11924/j.issn.1000-6850.2014-2495

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (20): 82-85.doi: 10.11924/j.issn.1000-6850.2014-2495

Special Issue: 园艺

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Detection of Cucumber Mosaic Virus Infecting Vegetable Crops Grown in Beijing Suburban Greenhouses

Chen Liu^1,2, Xing Dongmei³, Liu Zhengxiong³, Chen Xiaoyu⁴, Yang Jianqiang³, Hu Xuejun³, Shang Qiaoxia¹

(¹Beijing Key Laboratory of New Technology in Agricultural Application/ College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206; ²Niulanshan Second Primary School of Shunyi District, Beijing 101301;³Plant Protection and Quarantine Station of Changping District, Beijing 102200; ⁴Beijing Plant Protection Station, Beijing 100029)

Received:2014-09-13 Revised:2015-07-06 Accepted:2015-02-10 Online:2015-07-28 Published:2015-07-28

Abstract

Abstract: Cucumber mosaic virus (CMV) was detected by ELISA and RT-PCR methods to investigate the distribution and diversity of viruses infecting vegetable crops grown in Beijing suburban greenhouses. Leave samples of 10 vegetable crops were collected and then tested. Identical results were obtained by both ELISA and RT-PCR methods showed that 6 samples of celery, ornamental pumpkin, lettuce, peppers, eggplant and tomato ‘Dahongpao’ were positive while the other 4 samples of great pumpkin, brussels sprouts, four winged bean and tomato ‘Rome red’ were negative to CMV. In our study, the reliability of the results was confirmed by sequencing and sequence analysis of the RT-PCR products from the positive samples.

Chen Liu,Xing Dongmei,Liu Zhengxiong,Chen Xiaoyu,,Yang Jianqiang,Hu Xuejun and Shang Qiaoxia. Detection of Cucumber Mosaic Virus Infecting Vegetable Crops Grown in Beijing Suburban Greenhouses[J]. Chinese Agricultural Science Bulletin, 2015, 31(20): 82-85.

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Detection of Cucumber Mosaic Virus Infecting Vegetable Crops Grown in Beijing Suburban Greenhouses

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