Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2005, Vol. 21 ›› Issue (10): 368-368.

Special Issue: 水稻

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Preliminary Report on Transfer the Character of Drought Tolerance from Wild Rice (Oryza meridionalis and Oryza australiensis) to Cultivated Rice (Oryza sativa) via Distant Hybridization and Embryo Rescue

Chi Quanbo, Prapa Sripichitt, Xu Hongchun   

  • Online:2005-10-05 Published:2005-10-05

Abstract: It is well known that the lack of water in some areas of the world is threatening their agriculture, especially influences the growing and productivity of rice. So it is very important to breed new rice variety which has drought tolerance character or uses water economically. For example, in Beijing of China, rice harvesting area was very large in the past. Because of the lack of water, the available water is not enough for people to drink and not even more for rice planting. Consequently the area of rice planting in Beijing is decreasing every year. Up to now the area of rice planting is not over 1,000 hectares. There are diversified pattern of drought tolerance in Oryza spp.. If Oryza has drought tolerance trait, the cultivar would be environmental sound and economical viable. There would be great potential to increase the rice harvesting area and adaptability. The possible donors of character for drought tolerance are Oryza meridionalis and Oryza australiensis. In this experiment drought tolerance was transferred from wild rice species Oryza meridionalis and Oryza australiensis to cultivated rice Oryza Sativa through distant hybridization. The 2,419 florets of Oryza sativa were pollinated with Oryza meridionalis and 18 immature seeds of the F1 hybrid obtained were cultured for embryo rescue. Only one seed could germinate to be F1 hybrid plant. The plant set one F2 seed. The rate of crossability ranged from 0 to 0.46%, the rate of germination ranged from 0 to 50% and the rate of seed set of the F1 hybrid plant was 0.024%(1/4,211). The 1,848 florets of Oryza sativa were pollinated with Oryza australiensis and 48 immature seeds were obtained were cultured for embryo rescue. 11 of the 45 seeds could germinate to be F1 hybrid plants. They were all male sterile, the rate of crossability was 0.60% and the rate of germination was 31.1%. Morphological and Agronomic characters of the F1 hybrids of both crosses were observed. Finally advisable days after pollination for embryo rescue and “false” hybrid problem were discussed.