Abstract: In this study, a lower cost, time saving, simple operation and specificity dsRNA extraction method was explored to detect and identify the mycovirus in the main cultivated strains of Flammulina velutipes. We applied two different methods to extract the dsRNA in the main cultivated strains, extraction Kit method and Redzol method, and compared the advantages of these two methods. To identify the virus type, reverse transcription PCR was employed to amplify the dsRNAs and sequence a part of them. Results showed that both Kit and Redzol methods discovered the dsRNA in strain F-4889 and F-4891 of the tested strains. Compared with the Kit method, Redzol method was time saving, simple operation, cost saving and easy pretreatment, besides it just need small amount of samples. So it was more suitable for general laboratory and large to middle-size edible fungi factory. Redzol method can accurately extract dsRNA from the mycelium of F. velutipes, and detect FvBV virus in strain F-4889 and F-4891, which provide the theory basis for cultivation and breeding, and can optimize the germplasm resources of commercial strains.