Abstract: The study aimed to provide theoretical and technical basis for the local pepper culture in solar greenhouse by screening different substrate formulas. Using ‘Longjiao No.3’ pepper as test material, eight different substrate formulas was made by adding the different volume ratios of the fermented straw, sheep manure, cattle manure, mushroom residue, peat, vermiculite and river sand. The local traditional and commodity substrate were used as control to study the physical and chemical properties of different substrate formula and their effect on pepper fruit quality and yield. The results indicated that the formula G (corn stalk: cattle manure: peat: vermiculite: river sand=2:3.5:1:1:2.5), H (cornstalk: cattle manure: peat: river sand= 2:3.5:1:3.5), J (cornstalk: cattle manure: peat: vermiculite: river sand: mushroom residue= 2:2.5:1:1:2.5:1) showed excellent air capacity and water retention. The EC, bulk density, total porosity and the air water ratio were in optimum range; the quality of pepper fruit was excellent. The higher soluble sugar and Vc content and lower nitrate content was found in pepper fruit that planted in formula G, H and J. The yield of pepper planted in formula G, H and J was 60492.06, 59809.52 and 56939.68 kg/hm², respectively, significantly higher than that of pepper planted in formula A (traditional substrate). In summary, G, H and J formulation substrate exhibited excellent physical and chemical properties, good fruit quality and high yield, while the main raw material was from low cost agricultural waste and sand. Therefore, these three substrate formulations can be suitable for local extension.