Abstract: In order to improve the utilization rate of agriculture and forestry residues, and reduce consumption of peat resources, a pot experiment was carried out by using bamboo biochar from bamboo chip carbonization as alternative substrate partly replacing peat. A completely randomized block design was chosen in the experiment, with pine bark+ peat as the control group, and 3%, 6% and 10% of bamboo biochar substitution addition as the experimental group, respectively, which aimed to study the effects of different substrates on the growth of Dendrobium officinale. The results showed that there was no significant difference in yield, plant height, stem diameter, section spacing, leaf length, leaf width, root number and root length of Dendrobium officinalunder among 3% and 6% biochar alternative compared with the control treatment. However, the plant height, section spacing, section number, leaf number of Dendrobium officinale were significantly decreased with 10% amount of biochar alternative. Furthermore, the number of bud with 6% amount of biochar replacement was increased by 24.13%, while the survival rate was the lowest. Overall, in the short term, 3% amount of biochar alternative could be a new matrix to planting Dendrobium officinale, which can offer reference for the biochar utilization in crop cultivation, but the long-term effect about its utilization still needs to be further studied.